Figure 2.

SS-31 inhibits cyt c peroxidase activity. (A) Effects of various phospholipids on inducing cyt c peroxidase activity in vitro. PC, PE, PG, PS, or CL (30 μM) was added to 2 μM cyt c. Peroxidase activity was measured using amplex red fluorescence in the presence of 10 μM H₂O₂. (B) SS-31 dose-dependently inhibits cyt c peroxidase activity induced by 30 μM CL (EC₅₀=3.5±0.03 μM). (C) Effects of various phospholipids in inducing peroxidase activity in permeabilized mitochondria. (D) SS-31 dose-dependently inhibits CL-induced peroxidase activity in permeabilized mitochondria (EC₅₀=0.8±0.06 μM). The open square represents inhibition of peroxidase activity produced by 10 μM [ald]SS-31, showing that it has comparable activity with the activity of SS-31. (E) Effects of Ca²⁺ on CL-induced cyt c peroxidase activity. (F) SS-31 dose-dependently inhibits Ca²⁺-induced cyt c peroxidase activity (EC₅₀=0.8±0.06 μM). All data are represented as mean ± SEM (n=4–8).