Figure 5.

HIF-2α is required for EPO but not VEGF synthesis by CD133⁺/CD73⁺ cells. Quantitative RT-PCR analysis showing reduction in mRNA encoding for HIF-2α (A) and EPO (B), but not for VEGF (C) in CD133⁺ cells infected with two different shRNA for HIF-2α (shHIF2 alpha) in respect to cells infected with a scrambled vector (shCtr). Data are normalized to TBP mRNA and to 1 for shCtr and are the mean ± SD of three experiments performed using three different cell isolates. The t test is performed. *P<0.05 shHIF2 alpha versus shCtr. VEGF, vascular endothelial growth factor.