Figure 1.

Slit2 protein levels decrease in the kidney after IRI. Representative images of frozen kidney sections immunofluorescently labeled with anti-Slit2 antibody and Alexa 488 anti-goat IgG (green) from mice subjected to (A–C) sham surgery and (D–F) IRI. Original magnification, ×20. (A and D) DiI-conjugated wheat germ agglutinin staining (red) was performed to delineate cell membranes. (B and E) Immunolabeled Slit2 (green) and (C and F) merged images. White arrows indicate interstitial Slit2 staining. (G) Quantitative analysis of Slit2 levels using Volocity software was performed on 10 random, nonoverlapping 20× cortical fields sampled from sham surgery (n=3) and IRI kidneys (n=6) immunofluorescently labeled for Slit2. Data are presented as mean ± SEM. *P<0.05 versus sham surgery kidneys.