Figure 4.

Slit2 prevents renal dysfunction after IRI. (A) IRI was induced by bilateral cross-clamping of renal pedicles of male C57BL/6 mice for 26 minutes. Full-length hSlit2 (at the indicated doses) or vehicle was administered intraperitoneally before inducing IRI; 18 hours after reperfusion, blood was drawn by retro-orbital bleed, and plasma creatinine was determined using a colorimetric assay. (B) Truncated N-mSlit2 (2 µg) with or without molar equivalent amounts of RoboN was administered intraperitoneally before induction of renal IRI, and experiments were performed as in A. Mean values ± SEM from four to seven mice per experimental group. *P<0.05 versus sham-operated mice. ^†P<0.05 versus vehicle-treated IRI mice. ^‡P<0.05 versus hSlit2 1 µg-treated IRI mice. ^§P<0.05 versus N-mSlit2–treated IRI mice.