. Author manuscript; available in PMC: 2013 Aug 7.

Published in final edited form as: Microvasc Res. 2008 Dec 11;77(2):174–186. doi: 10.1016/j.mvr.2008.11.007

Table 1. Effects of pharmacological inhibitor pretreatments on PEG-induced endothelial barrier enhancement.

EC were pretreated with various agents (1μM S1P, 10μM FTY720, 100ng/ml PTX, Heparinase III for 4hr, or 10μM ML-7, 100nM Rho-kinase Inhibitor, 10μM U0126, 10μM LY294002, 10μM PP2, or 200μM Genistein for 30 min) and subsequently challenged with either 1μM S1P or 8% PEG to induced endothelial barrier enhancement. None of the agonists or inhibitors blocked PEG-induced barrier enhancement.

ECIS	S1P 24hr	FTY720 24hr	Heparinase III 4hr	PTX 4hr	ML-7/RhoK inhibitor/U0126/LY294002/PP2/Gen	PMA 24hr
S1P barrier enhancement	No effect	Blunted	-	Blocked	-	Blocked
PEG barrier enhancement	No effect	No effect	No effect	No effect	No effect	Affect only latter phase