Table 1.
Sequence of probes with alternating DNA/LNA monomer modification in this study. Bold italic letters indicates LNA monomers.
| Target Gene | Probe Type |  |
Length (base) | ΔGDQ (kcal/mol) | ΔGDT (kcal/mol) |
|---|---|---|---|---|---|
| β-actin | Donor (D) |  |
20 | −10.9 | −25.8 |
| Quencher (Q) |  |
10 | |||
| Target (T) | 5’-/CTCTTCCAGCCTTCCTTCCT/-3’ | 20 | |||
| HO-1 | Donor (D) |  |
20 | −11.8 | −26.6 |
| Quencher (Q) |  |
10 | |||
| Target (T) | 5’/AACAAGGAGAGCCCAGTCTT/3’ | 20 | |||
| HSP70 | Donor (D) |  |
20 | −10.2 | −24.9 |
| Quencher (Q) |  |
10 | |||
| Target (T) | 5’-/ATCACCATCACCAACGACAA/-3’ | 20 | |||
| Random | Donor (D) |  |
20 | −11.1 | −26.2 |
| Quencher (Q) |  |
10 | |||
| Target (T) | 5’/TATCGGTGCGCTTGTCGCGT/3’ | 20 |