Figure 3.

CARS microscopy of the mouse cornea. (A–F) show CARS (red) from individual coronal sections at various depths below the corneal surface indicated by the white lines in the corneal cross-section located above. CARS signal (red) from lipid membranes demonstrate the size and location of cells within the cornea. Larger cells in the corneal EP are seen closer to the surface ([A], 11 μm below surface of the cornea) compared with deeper within the EP ([B], 20 μm below). Keratinocytes/fibroblasts sparsely populate the corneal ST in (C–E) (38, 70, and 90 μm below the surface of the cornea). Cells of the EN appear as small structures between 3 and 5 μm ([F], 121 μm below). Scale bar: 25 μm.