Figure 5.

Transverse/sagittal section of the limbal region. In all panels, the cornea is oriented on the left with CARS signal shown in red and TPAF shown in green. (A) shows a line scan of the full thickness of mouse tissue centered on the limbal region of S and cornea. The collagen of the cornea (TPAF, green) is thicker in the cornea ST compared with the S, and the surface cell layers (CARS, red) of the corneal EP are thicker than the C. A higher resolution image of the limbus (boxed) is shown in (B), highlighting a “gap” between the ST and S that is sparsely populated by cells. A schematic of the tissue region is shown in (C), with arrows indicating the cells populating the “gap” of the limbus. Scale bar: 50 μm.