(A) Body weights of Ldlr−/− and Ldlr−/−: MYFKO mice fed WTD starting at 6 weeks of age. Intraperitoneal (B) glucose and (C) insulin tolerance tests in 14- or 15-week-old WTD-fed mice after 18-hr or 4-hr fast, respectively (n=8–10). Serum (D) glucose and (E) insulin levels in WTD-fed, 19-week-old mice fasted for 16 hr (0h, Fasted), or fasted for 16 hr and refed for 4 hr (Refed) (n=6–8). (F) Immunoblots of liver insulin signaling of 14-week-old mice fed WTD for 8 weeks. After a 16-hr fast, mice were injected with insulin or PBS and livers were collected 3 min later (n=3). (G) Liver gene expression in 14-week-old Ldlr−/− and Ldlr−/−: MYFKO mice fed WTD for 8 weeks and fasted for 16 hr (n=6–8). * p < 0.05, ** p < 0.01 vs. Ldlr−/−.