Figure 3. Interactions of SERCA2b and Bcl-2 in C2C12 myoblast cells.

C2C12 myoblasts were transfected with empty vector (control), or with Bcl-2 and Bcl-cb5 DNA followed by IP at 24 h after transfection: A,B - IP with anti-SERCA2 antibody and WB analysis for Bcl-2; C - IP with anti-Bcl-2 antibody and WB analysis for SERCA2; D – WB analysis for Bcl-2 in total lysate (1) and in the microsomal fraction of non-transfected C2C12 cells after IP with anti-SERCA2 antibodies (2). In A–D, protein bands were obtained in the same blots after which lanes were merged for comparison. Input data are shown for source samples prior to IP. E – Effect of Bcl-2 overexpression on thapsigargin-inhibitable ⁴⁵Ca²⁺ uptake by C2C12 microsomes. For Bcl-2 overexpressing cells, average and experimental error are calculated from the data of 4 independent experiments.