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A – WB analysis for SERCA2b immunoprecipitated from HEK293 cell microsomes using anti-Bcl-2 antibodies (1) or protein A beads only (2); the two bands represent SERCA2b monomer and aggregate. B – WB analysis for Bcl-2 in microsomes of HEK293 cells transfected with empty vector (1) or Bcl-2 (2). C – Lack of the effect of Bcl-2 overexpression on Ca2+ uptake by microsomes isolated from HEK293 cells.
