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. Author manuscript; available in PMC: 2013 Aug 7.
Published in final edited form as: Biochem J. 2012 May 15;444(1):127–139. doi: 10.1042/BJ20111114

Figure 6. HSP70 and GroEL attenuate Bcl-2Δ21-dependent inhibition of Ca2+-ATPase activity of SERCA1.

Figure 6

SR (0.5 mg/ml protein) was incubated in STE buffer containing 1 mM PMSF at 37°C in the absence or presence of Bcl-2Δ21 (at a molar ratio of [Bcl-2Δ21]/[SERCA]=2), HSP70 or GroEL (0.25 mg/ml each). The aliquots were analyzed for Ca2+-ATPase activity after 2 h of incubation as described under “Experimental Procedures”.