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. Author manuscript; available in PMC: 2013 Aug 7.
Published in final edited form as: Biochem J. 2012 May 15;444(1):127–139. doi: 10.1042/BJ20111114

Figure 7. Effect of HSP70 on sucrose gradient fractionation of skeletal muscle SR.

Figure 7

SR (20 µg of total protein) was incubated at 37°C in STE buffer containing 1 mM PMSF for 1 h (A) without additions, (B) with Bcl-2Δ21 at a molar ratio [Bcl-2Δ21]/[SERCA1] =2, (C) in the presence of 10 µg of HSP70, and (D and E) in the presence of 10 µg of HSP70 and Bcl-2Δ21 at a molar ratio [Bcl-2Δ21]/[SERCA1] =2. After sucrose density gradient separation, fractions were collected from the top of the gradient, separated by SDS-PAGE, and analyzed by WB with anti-SERCA1 (A–D) or anti-HSP70 (E) antibody. Fraction numbers are indicated at respective lanes.