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. Author manuscript; available in PMC: 2014 May 28.
Published in final edited form as: Dev Cell. 2013 May 16;25(4):402–416. doi: 10.1016/j.devcel.2013.04.011

Figure 3. MT1-MMP Regulates SSC Commitment and Differentiation.

Figure 3

(A) Bone-marrow-derived SSCs were isolated from Mmp14+/+ and Mmp14−/− mice and cultured under osteogenic conditions for 7 days (alkaline phosphatase stained) or 14 days (alizarin red S stained) in 3D collagen, under adipogenic conditions in 3D collagen for 7 days (oil red O stained), or under chondrogenic conditions for 21 days (safranin O/fast green stained). The scale bars represent 100 μm.

(B) Relative mRNA expression of osteogenic, adipogenic, and chondrogenic markers from the cultures shown in (A) (n = 3). Data are presented as mean ± SEM. **p < 0.01, unpaired t test.

(C) Schematic of the overall experimental design for in vivo implantation.

(D) Histology of tissues isolated from mice transplanted with Mmp14+/+ or Mmp14−/− SSCs. The scale bars represent 100 μm (n = 5).

(E) Experimental design of chondrogenesis in vivo.

(F) Safranin O staining and chondrogenic marker expression levels of cartilage-like tissues isolated from mice transplanted with Mmp14+/+ or Mmp14−/− SSCs cultured as described in (E). The scale bar represents 100 μm (n = 5). Data are presented as mean ± SEM. **p < 0.01, unpaired t test.

See also Figure S3.