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. Author manuscript; available in PMC: 2013 Aug 7.
Published in final edited form as: Oncogene. 2012 Mar 5;32(3):388–396. doi: 10.1038/onc.2012.63

Figure 1. RPS14 interacts with MDM2 in cells.

Figure 1

(A) (B) Exogenous RPS14 interacts with exogenous MDM2 in 293 cells. Cells were transfected with HA-MDM2, Flag-RPS14, or Flag-RPS19 plasmids and harvested for IP (0.5 mg total proteins) using anti-Flag, anti-HA, or mouse IgG, followed by IB with the antibodies as indicated. (C) Endogenous RPS14 interacts with endogenous MDM2 in HCT116 cells. Cells lysates (1 mg) were prepared from HCT116 cells after treatment with Act D for 8 h. IP was conducted with anti-RPS14 or rabbit IgG followed by IB with anti-MDM2 or anti-RPS14. (D) Exogenous RPS14 does not interact with exogenous MDMX in H1299 cells. Cells were transfected with combinations of HA-MDM2, Myc-MDMX, Flag-S14 or Flag-L11 plasmids and harvested for co-IP-IB assays using the antibodies as indicated. All the experiments were repeated more than 2 times and true to the rest of data.