Figure 5.

Inflammatory cytokine expression is increased in twi-5J nervous system. Quantitative RT–PCR for IL1-β and LIF expression was performed on RNA samples processed from the cortex (CTX), brainstem (BS), spinal cord (SpC) and sciatic nerve (ScN) of wild-type and twi-5J at P7, P15 or P25 (mean ± SEM, n = 3). (A) IL1-β and LIF expression is significantly increased in the CNS at P25, but not at P15. (B) In the sciatic nerve, IL1-β expression is significantly elevated and progressively increases starting at P7, while LIF expression is not increased in twi-5J compared with wild-type. *P < 0.05; **P < 0.01; ***P < 0.005.