Figure 1.

Increased cholesterol promotes Aβ synthesis and loading. (A) β-Secretase activity analyzed in brain extracts from WT and transgenic mice (AU: arbitrary units). *P < 0.05, **P < 0.01 (n = 3). Values are expressed as mean ± SD (B) Representative photomicrographs of hippocampus labeled with human anti-Aβ(1-42) and counterstained with hematoxylin showing early Aβ depositions in APP/PS1/SREBP-2 mice. Scale bar: 100 μm. (C) Brain from WT and indicated mutant mice (A/P: APP/PS1 mice; A/P/S-2: APP/PS1/SREBP-2 mice) were homogenized with guanidine HCl extraction buffer, and the homogenates were analyzed by ELISAs for quantitative assessment of the human Aβ(1-42) content at the indicated ages (n = 6 per genotype).