Figure 3.
Loss of Mbnl3 expression inhibits C2C12 myogenic differentiation. (A) C2C12 myoblasts were grown on plates supplemented with Matrigel, transfected with either non-targeting (siRNAnt) or Mbnl3 (siMbnl3) siRNA pools, and then induced to differentiate 24 h post-siRNA treatment. Mbnl3, Mbnl1 and Celf1 protein levels were monitored by immunoblot analysis for 0–72 h post-induction and differentiation status was assessed using antibodies against myogenin (Myog) and Myhc. (B) Cell immunofluorescence analysis of C2C12 myotube formation showing impaired myogenic differentiation at 48 and 72 h after transfer to differentiation media following siMbnl3-induced knockdown compared with siRNAnt control (scale bar = 50 μM). (C) Myotube number (>2 myonuclei/myotube per field) decreases following Mbnl3 depletion. Data are SEM and significant (**P < 0.01).