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. 2013 Jul 25;11(1):8. doi: 10.1186/1897-4287-11-8

Figure 3.

Figure 3

PTEN protein analysis. Representative image of three repeated experiments qualitatively similar. A)Western blot assay of PTEN performed on protein extracts from peripheral blood cells. The amounts of PTEN and β-actin were measured by Western blot. II-3, III-4, III-3, III-2, III-1: patients-numbering corresponds to that adopted in the shown pedigree. B)Histogram showing optical density of PTEN protein normalized versus actin protein: Density of the electrophoretic band was obtained with ImageJ software. Relative quantification of PTEN protein normalized versus actin protein was calculated using sample III-3 as calibrator. C)Western blot assay of PTEN performed on protein extracts from colon mucosa. N2: healthy colon mucosa and P2: amartomatous polyp of proband’s daughter described in case 2; N3: healthy colon mucosa and T3: colorectal carcinoma of proband 3; C: healthy colon mucosa. D)Histogram showing optical density of PTEN protein normalized versus actin protein: Density of the electrophoretic band was obtained with ImageJ software. Relative quantification of PTEN protein normalized versus actin protein was calculated using sample N-2 as calibrator.