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. Author manuscript; available in PMC: 2014 Apr 9.

Published in final edited form as: J Proteomics. 2013 Feb 4;81:173–184. doi: 10.1016/j.jprot.2013.01.026

Inhibiting the Propagation of False Annotations using the Noise Control Method. A small number of falsely annotated spectra are present in peptide spectral libraries. A. As shown in the example, a high correlation between the query spectrum and the falsely annotated reference spectrum (Q8BW94: EISLYSMGFLDSRSLAQK) (DP = 0.751) accompanied a high noise correlation value (NDP = 0.277). The adjustment of the spectral correlation score (ΔDP=DP−NDP=0.474) assured the accuracy of spectral analysis; thus inhibiting the propagation of this false annotation. B. With a correctly annotated reference spectrum (Q6P8J7: LSEMTEQDQQR), the difference in dot product values (ΔDP=0.683−0.111=0.572) supported correct spectral annotation.

Inhibiting the Propagation of False Annotations using the Noise Control Method. A small number of falsely annotated spectra are present in peptide spectral libraries. A. As shown in the example, a high correlation between the query spectrum and the falsely annotated reference spectrum (Q8BW94: EISLYSMGFLDSRSLAQK) (DP = 0.751) accompanied a high noise correlation value (NDP = 0.277). The adjustment of the spectral correlation score (ΔDP=DP−NDP=0.474) assured the accuracy of spectral analysis; thus inhibiting the propagation of this false annotation. B. With a correctly annotated reference spectrum (Q6P8J7: LSEMTEQDQQR), the difference in dot product values (ΔDP=0.683−0.111=0.572) supported correct spectral annotation.