Figure 4. Identification of CUEs as a PCBP1-binding site in p63 3′UTR.

(A) Schematic presentation of p63α transcript and the location of probes. (B) REMSA was performed by mixing ³²P-labeled RNA probes (A, A1, or A2) with recombinant GST and GST-PCBP1proteins, respectively. (C) REMSA assay was performed by mixing a ³²P-labeled RNA probe A1 along with or without excess amount (50-fold) of unlabeled probe A1 or A2. (D) REMSA was performed by mixing a ³²P-labeled RNA probe (A1, CG1, or CG2) with recombinant GST or GST-fused PCBP1.