Skip to main content
. 2013 Aug 7;8(8):e71130. doi: 10.1371/journal.pone.0071130

Figure 2. The effects of EF24 on STAT and NF-κB activation in vitro.

Figure 2

DU145 (A,B) and B16 (D,E) cells were treated with EF24 (5 µM) or vehicle (control) for 24 hr prior to stimulation with IFN (1000 IU/ml) for the indicated times. Whole cell extracts were prepared and immunoblotted for (A,D) IκB and actin, or (B,E) phosph-STAT3 and STAT3. (C,F) Nuclear extracts were prepared from IFN-stimulated cells (1000 IU/ml for 30 min) DU145 (C) or B16 (F) cells pretreated for 24 hr with EF24 (5 µM) or vehicle (control) and subjected to EMSA with oligonucleotide probes for NF-κB or SIE. A 50-fold excess of unlabeled probe (cold) was used to show the specificity of binding. Representative results from at least three experiments are shown.