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. 2013 Aug 7;8(8):e71130. doi: 10.1371/journal.pone.0071130

Figure 9. The effects of EF24 on miRNA expression in vitro and in vivo.

Figure 9

(A) Total RNA was prepared from DU145 cells treated with vehicle and EF24 (5 µM, 24 hr), or tumor tissue from DU145 xenografts of control and EF24-treated mice (200 µg/kg body weight) at autopsy, and miRNA expression profiling was conducted on the nCounter Analysis System using the human V1 miRNA assay kit. (B) Venn diagram analysis of the genes upregulated or downregulated by EF24 treatment in cells and tumor tissue. (C) PCR validation of the genes upregulated or downregulated by EF24 treatment in both cells and tumor tissue (n = 3), and expressed as relative gene expression (EF24-treated/control).