Figure 4.

A general lack of dosage compensation observed in HeLa gene expression. (A) Correlation between CN and gene expression levels. Empirical cumulative distribution functions of gene expression values (for genes detected as expressed), grouped by CN state of the region containing the gene. The x-axis shows the logarithm (base 10) of read counts per kb of gene and the y-axis shows the corresponding cumulative distribution function. Significance (*) was calculated by the Wilcoxon test (P < 0.01). (B) Lack of allele-specific dosage compensation. For each SNV in genome segments of CN 3, the higher and lower RNA-Seq read count for both alleles are shown (higher count on the y-axis, lower count on the x-axis). The two dashed lines represent ratios 2:1 and 1:1. The observed ratios center around the 2:1 line, indicating an overall lack of allele-specific dosage compensation.