Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Aug 1;3(8):1261–1272. doi: 10.1534/g3.113.006213

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2013 Terweij et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution Unported License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Immunoblot and ChIP analysis of histone H3 dynamics. (A) Histone H3 was tagged with a V5→HA-6xHis RITE cassette in strain NKI8050. (B) Outline of experimental setup. (C) Immunoblot analysis of cells arrested by starvation (pre), switched for 16 hr (post), and subsequently released into fresh media for 2 hr (no cell division) or 4 hr (one cell division). An antibody against spacer-LoxP detects both old and new H3. (D-E) H3-V5 and H3-HA-6xHis ChIP analysis of samples described in (B) to measure occupancy (IP/input) of old and new H3 at PTC6 (around transcription start site) and SPA2 (coding sequence) (primer sequences are listed in Table 3). (F) Exchange of histone H3 determined as the ChIP ratio of new H3 over old H3 (HA/V5). Average of three biological replicates ± SEM.