Table 1. Yeast strains.
| Name | Relevant Genotype | Switch Parent | Sourcea |
|---|---|---|---|
| NKI2036b | MATa his3Δ1 leu2Δ0 lys2Δ0 met15Δ0 ura3Δ0 hhf1-hht1Δ::LEU2 | KV | |
| NKI2148 | NKI2036; bar1Δ::HisG his3Δ1::HIS3-PTDH3-CRE-EBD78 hht2::HHT2-LoxP-HA-TADH1-HphMX-LoxP-T7 | KV | |
| NKI8085 | NKI2036; hht2::HHT2-LoxP-T7 | NKI2148 | This study |
| NKI2158 | NKI2036; TCYC1::PTDH3-CRE-EBD78-HIS3 hht2::HHT2-LoxP-T7-TADH1-HphMX-LoxP-HA | KV | |
| NKI4138 | NKI2036; hht2::HHT2-LoxP-HA | NKI2158 | This study |
| BY4733 | MATa his3Δ200 leu2Δ0 met15Δ0 trp1Δ63 ura3Δ0 | CB | |
| NKI8001 | BY4733; bar1Δ::HISG his3::HIS3-PTDH3-CRE-EBD78 | This study | |
| NKI2176 | BY4733; hht1-hhf1::MET15 bar1Δ::HisG his3Δ200::HIS3-PTDH3-CRE-EBD78 | This study | |
| NKI2178 | NKI2176; hht2::HHT2-LoxP-HA-6xHis-TADH1-HphMX-LoxP-T7 | This study | |
| NKI8086 | NKI2176; hht2::HHT2-LoxP-T7 | NKI2178 | This study |
| NKI2220 | NKI2176; hht2::HHT2-LoxP-T7- TADH1-HphMX-LoxP-HA-6xHis | This study | |
| NKI8037 | NKI2220; hht2::HHT2-LoxP-HA-6xHis | NKI2220 | This study |
| NKI8051 | NKI2176; hht2::HHT2-LoxP-2xT7- TADH1-HphMX-LoxP-HA-6xHis | This study | |
| NKI8088 | NKI8051; hht2::HHT2-LoxP-HA-6xHis | NKI8051 | This study |
| NKI8056 | NKI2176; hht2::HHT2-LoxP-HA-6xHIS- TADH1-HphMX-LoxP-V5 | This study | |
| NKI8058 | NKI8056; hht2::HHT2-LoxP-V5 | NKI8056 | This study |
| NKI8050 | NKI2176; hht2::HHT2-LoxP-V5- TADH1-HphMX-LoxP-HA-6xHis | This study | |
| NKI8087 | NKI8050; hht2::HHT2-LoxP-HA-6xHis | NKI8050 | This study |
| NKI8052 | NKI2176; hht2::HHT2-LoxP-2xFLAG- TADH1-HphMX-LoxP-HA-6xHis | This study | |
| NKI8089 | NKI8052; hht2::HHT2-LoxP-HA-6xHis | NKI8052 | This study |
| NKI8030 | NKI8001; htz1::HTZ1-LoxP-HA-6xHIS-TADH1-HphMX-LoxP-T7 | ||
| NKI8053 | NKI8001; htz1::HTZ1-LoxP-V5-TADH1-HphMX-LoxP-HA-6xHIS | ||
| NKI8054 | NKI8001; htz1::HTZ1-LoxP-2xT7-TADH1-HphMX-LoxP-HA-6xHIS | ||
| NKI8055 | NKI8001; htz1::HTZ1-LoxP-2xFLAG-TADH1-HphMX-LoxP-HA-6xHIS | ||
| NKI4044 | BY4733; pgk1::PGK1-V5-LoxP-HA-yEGFP- TADH1-HphMX-LoxP-T7-mRFP | This study | |
| NKI4044post | NKI4044; pgk1::PGK1-LoxP-T7-mRFP | NKI4044 | This study |
| Y7092 | MATα can1Δ::PSTE2-Sp-his5 lyp1Δ his3Δ1 leu2Δ0 ura3Δ0 met15Δ0 | AT | |
| SPC42G | Y7092; lyp1Δ::NATMX-PTDH3-CRE-EBD78 spc42::SPC42-loxP-T7- TADH1-HphMX-loxP-HA-GFP | This study | |
| SPC42Gpost | SPC42G; spc42::SPC42-loxP-HA-GFP | SPC42G | This study |
| SPC42R | Y7092; lyp1Δ::NATMX-PTDH3-CRE-EBD78 spc42::SPC42-LoxP-3xHA- TADH1-HphMX-LoxP-3xT7-mRFP | This study | |
| SPC42Rpost | SPC42R; spc42::SPC42-LoxP-3xT7-mRFP | SPC42R | This study |
a
Sources: Verzijlbergen et al. 2010; Brachmann et al. 1998; Tong et al. 2006; van Leeuwen et al. 2002, see Materials and Methods.
b
NKI2036 was derived from crosses between BY4742, BY4727, and UCC1369 (CB and FvL).