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. 2013 Aug 1;3(8):1261–1272. doi: 10.1534/g3.113.006213

Table 2. Oligos used for plasmid construction.

Oligo Sequence
CLTSB19a GTACTATGGCTTCTATGACAGGAGGTCAACAGATGGGAGGAATGGCCTCAATGACCGGTGGCCAGCAAATGGGAT
CLTSB19b GTACATCCCATTTGCTGGCCACCGGTCATTGAGGCCATTCCTCCCATCTGTTGACCTCCTGTCATAGAAGCCATA
CLTSB17a CTAGCTATCCCTATGACGTCCCGGACTATGCAGGATATCCATATGACGTTCCAGATTACGCTA
CLTSB17b CTAGTAGCGTAATCTGGAACGTCATATGGATATCCTGCATAGTCCGGGACGTCATAGGGATAG
KpnI-V5-SpeI Fwd CGGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTA
KpnI-V5-SpeI Rev CTAGTAGAATCGAGACCGAGGAGAGGGTTAGGGATAGGCTTACCGGTAC
KpnI-2xFLAG-SpeI Fwd CGACTACAAGGACGATGACGATAAAGATTATAAAGATGACGATGACAAGA
KpnI-2xFLAG-SpeI Rev CTAGTCTTGTCATCGTCATCTTTATAATCTTTATCGTCATCGTCCTTGTAGTCGGTAC
KpnI-2xT7-SpeI Fwd CATGGCTTCTATGACAGGAGGTCAACAGATGATGGCAAGCATGACTGGTGGACAGCAAATGA
KpnI-2xT7-SpeI Rev CTAGTCATTTGCTGTCCACCAGTCATGCTTGCCATCATCTGTTGACCTCCTGTCATAGAAGCCATGGTAC

Fwd, forward, Rev, reverse.