Table 2. Oligos used for plasmid construction.
Oligo | Sequence |
---|---|
CLTSB19a | GTACTATGGCTTCTATGACAGGAGGTCAACAGATGGGAGGAATGGCCTCAATGACCGGTGGCCAGCAAATGGGAT |
CLTSB19b | GTACATCCCATTTGCTGGCCACCGGTCATTGAGGCCATTCCTCCCATCTGTTGACCTCCTGTCATAGAAGCCATA |
CLTSB17a | CTAGCTATCCCTATGACGTCCCGGACTATGCAGGATATCCATATGACGTTCCAGATTACGCTA |
CLTSB17b | CTAGTAGCGTAATCTGGAACGTCATATGGATATCCTGCATAGTCCGGGACGTCATAGGGATAG |
KpnI-V5-SpeI Fwd | CGGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTA |
KpnI-V5-SpeI Rev | CTAGTAGAATCGAGACCGAGGAGAGGGTTAGGGATAGGCTTACCGGTAC |
KpnI-2xFLAG-SpeI Fwd | CGACTACAAGGACGATGACGATAAAGATTATAAAGATGACGATGACAAGA |
KpnI-2xFLAG-SpeI Rev | CTAGTCTTGTCATCGTCATCTTTATAATCTTTATCGTCATCGTCCTTGTAGTCGGTAC |
KpnI-2xT7-SpeI Fwd | CATGGCTTCTATGACAGGAGGTCAACAGATGATGGCAAGCATGACTGGTGGACAGCAAATGA |
KpnI-2xT7-SpeI Rev | CTAGTCATTTGCTGTCCACCAGTCATGCTTGCCATCATCTGTTGACCTCCTGTCATAGAAGCCATGGTAC |
Fwd, forward, Rev, reverse.