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. 2013 Aug 1;3(8):1363–1374. doi: 10.1534/g3.113.006999

Figure 5.

Figure 5

π fate specification defects in hda-1 animals. Animal stages and transgenes are shown on the lateral side of the images and genotypes on the bottom of each image. Arrowheads mark the center of vulval invagination. π cells and their progeny are indicated by asterisks. (A, B) In a wild-type egl-13::gfp L4 animal, 7 gfp-expressing cells (6 π progeny and the AC) are visible. (E, F) A lin-11::gfp animal of similar age shows 6 π progeny in this focal plane. (C, D) hda-1 RNAi causes an increase in π cells. An egl-13::gfp animal showing 10 π progeny following hda-1 knockdown. (G, H) Similar knockdown in a lin-11::gfp strain results in significant reduction in GFP fluorescence in vulval cells. The π progeny in this animal are too faint to see. (I, J) The e1795 allele of hda-1 causes greater reduction in lin-11:gfp expression. In this animal, no fluorescence is visible in the vulva or uterine cells. π cells in egl-13::gfp (K, L) and lin-11::gfp (O, P) animals. (M, N and Q, R) An increased number of π cells are observed in egl-13::gfp and lin-11::gfp animals following hda-1 knockdown. (S) Quantification of egl-13::gfp and lin-11::gfp expressing cells in late-L3 and early/mid-L4 stage animals. The percentage of animals is shown on the x-axis, whereas genotypes are indicated on the y-axis. N = number of animals examined; Scale bar (A−R) is 10 μm.