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. 2013 Aug 1;3(8):1363–1374. doi: 10.1534/g3.113.006999

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Copyright © 2013 Ranawade et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution Unported License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Effect of hda-1(RNAi) on AC expression of transcription factors. Transgenic animals with fluorescent reporters for lin-29 (A, B), hlh-2 (C, D), nhr-67 (E, F), and egl-43 (G, H) were treated with either control L4440 or hda-1 RNAi. Nomarski images are on the left, and the corresponding fluorescence images are on the right. GFP fluorescence is unaltered in lin-29::wcherry and hlh-2::gfp animals. However, nhr-67::wcherry and egl-43::gfp fluorescence in the AC is reduced. lin-29::wcherry expression is also observed in vulval lineage cells. Arrowheads mark the AC and the star in G points to a VU cell. 20 or more animals were examined in each case. Scale bar is 5 μm.