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. 2013 Aug 1;3(8):1399–1407. doi: 10.1534/g3.113.006130

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Copyright © 2013 Howlett et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution Unported License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Suppression of para^bss1/+ BS paralytic phenotype by a heterozygous chromosomal segment deleted in 89B. (A) Depicted is polytene chromosome map of region 89 on 3R. (B) The segment deleted in Df(3R)ED10639 causes suppression of para^bss1/+ BS paralysis, as described in the text. Also, Df(3R)Exel7329 causes suppression but Df(3R)Exel6269 does not. The breakpoints of these rearrangements delimit a small region (89B9 to 89B12) responsible for seizure suppression. (C) Six genes are contained in the 89B9 to 89B12 chromosomal segment including tara, bor, and gish. (D) BS paralytic phenotypes (% BS paralysis) of several genotypes in a para^bss1/+ background, as described in the text. Genotypes showing BS suppression are depicted as black bars; gray bars are used in genotypes showing no suppression. In each case, the experimental genotype shown is normalized relative to sibling controls. Df ED10639 is the genotype para^bss1/+; Df(3R) ED10639/+ showing 13% BS paralysis (87% suppression of BS phenotype). This indicates the apparent presence of a gene that acts as a haplo-seizure suppressor. Df Exel7329 is para^bss1/+;Df(3R)Exel7329/+ showing 13% BS paralysis and providing one boundary for suppressor location at 89B9 based on inclusion within the deleted segment. Df Exel6269 is para^bss1/+;Df(3R)Exel6269/+ showing 100% BS paralysis and providing a second boundary for suppressor location at 89B12 based its exclusion from the deletion. Flies that are para^bss1/+;bor^c05496/+ and para^bss1/+;tara¹/+ (labeled bor and tara) show no suppression with 91% and 100% BS paralysis, respectively. Flies that are para^bss1/+;gish⁰⁴⁸⁹⁵/+ (labeled gish⁰⁴⁸⁹⁵) show 43% BS paralysis, indicating suppression of the BS paralytic phenotype. Flies that are para^bss1/+;gish^EX04895/+ (labeled gish^EX04895) are a line with a remobilized, precise excision of the gish^EX04895 P-element; they show no suppression with 98% BS paralysis. Flies that are ELAV-Gal4^C155 para^bss1/+; UAS-gishRNAi/+ (labeled ELAV-GAL4) show 25% BS paralysis indicating suppression of the BS paralytic phenotype. Several gish alleles as heterozygotes show no suppression of para^bss1/+ BS paralytic phenotypes. Thus, gish^e01759/+, gish^DG16412/+, gish^KG03891/+, gish^EY06457/+ heterozygous combinations in a para^bss1/+ background show 95%, 88%, 84%, and 83% BS paralysis, respectively.