Figure 5. Chorioallantoic membrane (CAM) assay.

At day 9 of embryonic development, matrigel droplets with or without 50,000 hDPSC were applied onto the CAM of a chicken embryo (black arrow, Figure 5a). 72 hours later, pictures were taken (figure 5c–e). Figure 5c is a control matrigel droplet, while Figure 5d is a droplet containing 50,000 hDPSC. White scale bars represent 1 mm. To assess angiogenesis, two circles (with a radius of 1.5 and 2 mm, see figure 5e), were digitally positioned around the matrigel droplets and the blood vessels intersecting these circles were counted double blind. Graph 5b: hDPSC were able to significantly increase the number of capillaries intersecting both circles. This assay was repeated 4 times with hDPSC of 4 different donors to gain a database of at least 27 individual eggs. Values are represented as means ± S.E.M.