Figure 5. C. albicans CDR4 transcript levels grown in the presence of bacteria.

C. albicans SC5314, E. coli, P. aeruginosa, and S. aureus were grown to mid log phase in liquid culture, mixed together in equal amounts, co-incubated at 37° C, and aliquots were taken at 0, 10, 20, 30, and 60 minutes post addition. RNA was isolated and expression of CDR4 was measured by reverse transcription. ENO1 was used as a loading control and reference gene for expression comparisons. Graphical representation of CDR4 expression over time for SC5314 co-incubated with E. coli, P. aeruginosa, or S. aureus. Data is representative of three independent experiments with mean value and standard deviation bars shown. The asterisks indicate a statistically significant difference (P<0.05) in mean intensity of test conditions over the control.