Table 1. Eprobes used for this study.
| Eprobe Name | Length(bases) | Dye(nm) | TM (°C)(full match) | TM (°C)(mismatch) | Sequence |
| Eprobe 203-10 wt TO | 10 | D514 | 52.5 | 39.7 | TTZGGGCTGG |
| DNA/DNA | 10 | – | 36.6 | 19.4 | TTTGGGCTGG |
| Eprobe 205-13 wt TO | 13 | D514 | 63.3 | 54.0 | TTTZGGGCTGGCC |
| DNA/DNA | 13 | – | 51.7 | 40.3 | TTTTGGGCTGGCC |
| Eprobe205-13m TO | 13 | D514 | 67.0 | 56.7 | TTTZGGGCGGGCC |
| DNA/DNA | 13 | – | 55.8 | 43.4 | TTTTGGGCGGGCC |
| Eprobe 215-21 wt TO | 21 | D514 | 77.2 | 72.5 | TTGGGCTGGCCAAACZGCTGG |
| DNA/DNA | 21 | – | 68.9 | 63.3 | TTGGGCTGGCCAAACTGCTGG |
| Eprobe 215-21 wt TP | 21 | D570 | 65.2* | 57.2* | TTGGGCTGGCCAAACZGCTGG |
| Eprobe 215-21m TO | 21 | D514 | 79.5 | 74.0 | TTGGGCGGGCCAAACZGCTGG |
| DNA/DNA | 21 | – | 71.3 | 65.0 | TTGGGCGGGCCAAACTGCTGG |
| Eprobe KWT14m3 TO | 14 | 64.8 | – | AGCTGGTGGCGZAG | |
| DNA/DNA | 14 | D514 | 54.4 | – | AGCTGGTGGCGTAG |
(TM values for TO-labeled Eprobes were estimated using the following settings: 0.05 M Na+, 0.002 M Mg2+, 0.2 µM Eprobe, 60°C Temperature; *TM values for Eprobe 215-21 wt TP are actual values; in Eprobe name: TO = D514, TP = D570; Z indicating position of modified T).