Table 3. Temperature values of melting curve peaks of GPM6B (A), MAGEA12 (B) and FCRL1 (C) amplicons in tumor samples and normal liver tissues in Chinese (Ch) and Bangladeshi (B) cohorts as analyzed by HRM.
| GPM6B | MAGEA12 | FCRL1 | ||||||||||||||
| HCC | NorAdj/CHB | HCC | NorAdj/CHB | HCC | NorAdj/CHB | |||||||||||
| AVG | SD | AVG | SD | P | AVG | SD | AVG | SD | P | AVG | SD | AVG | SD | P | ||
| Ch | Patient 1 (PVTT) | 73.91 | 0.04 | 74.62 | 0.10 | <0.0001 | 74.21 | 0.13 | 75.41 | 0.21 | <0.0001 | 72.46 | 0.22 | 72.51 | 0.35 | 0.016 |
| Patient 4 (PVTT) | 74.04 | 0.05 | 74.59 | 0.15 | <0.0001 | 74.18 | 0.02 | 75.38 | 0.26 | <0.0001 | 72.49 | 0.17 | 72.27 | 0.19 | 0.018 | |
| Patient 5 (PVTT) | 74.49 | 0.01 | 74.49 | 0.01 | 0.018 | 75.62 | 0.01 | 75.40 | 0.15 | 0.166 | 72.49 | 0.18 | 72.54 | 0.20 | 0.018 | |
| Patient 6 (PVTT) | 74.19 | 0.13 | 74.56 | 0.11 | 0.0002 | 75.48 | 0.21 | 75.48 | 0.11 | 0.58 | 72.58 | 0.05 | 72.49 | 0.17 | 0.031 | |
| Patient 7 (PVTT) | 73.84 | 0.06 | 74.60 | 0.06 | <0.0001 | 75.72 | 0.03 | 75.60 | 0.05 | 0.06 | 72.28 | 0.27 | 72.68 | 0.00 | 0.0056 | |
| Patient 8 | 73.80 | 0.01 | 74.63 | 0.08 | <0.0001 | 74.26 | 0.20 | 75.43 | 0.29 | 0.0002 | 72.97 | 0.03 | 73.16 | 0.10 | <0.0001 | |
| Patient 9 | 73.83 | 0.14 | 74.63 | 0.08 | <0.0001 | 74.49 | 0.02 | 75.42 | 0.32 | 0.0006 | 72.58 | 0.54 | 72.75 | 0.68 | 0.084 | |
| Patient 10 | 73.80 | 0.01 | 74.63 | 0.01 | <0.0001 | 74.95 | 0.20 | 75.26 | 0.04 | 0.032 | 72.66 | 0.51 | 73.23 | 0.00 | 0.13 | |
| Patient 11 | 73.81 | 0.11 | 74.63 | 0.08 | <0.0001 | 74.70 | 0.02 | 75.40 | 0.24 | 0.0027 | 72.98 | 0.02 | 73.30 | 0.00 | 0.61 | |
| Patient 12 | 73.80 | 0.19 | 74.67 | 0.04 | <0.0001 | 74.45 | 0.07 | 75.43 | 0.20 | 0.0005 | 73.01 | 0.02 | 73.16 | 0.20 | 0.73 | |
| Patient 14 | 73.91 | 0.06 | 74.63 | 0.08 | <0.0001 | 74.52 | 0.03 | 75.40 | 0.24 | 0.0008 | 72.80 | 0.71 | 72.75 | 0.64 | 0.36 | |
| Patient 15 | 74.08 | 0.08 | 74.63 | 0.08 | <0.0001 | 74.66 | 0.03 | 75.37 | 0.19 | 0.0021 | 72.70 | 0.56 | 73.15 | 0.12 | 0.17 | |
| All HCC Average | 73.96 | 0.03 | 74.61 | 0.00 | <0.0001 | 74.77 | 0.03 | 75.41 | 0.19 | <0.0001 | 72.66 | 0.26 | 72.83 | 0.22 | 0.0028 | |
| Non-PVTT Average | 73.86 | 0.06 | 74.64 | 0.06 | <0.0001 | 74.57 | 0.06 | 75.39 | 0.21 | <0.0001 | 72.81 | 0.33 | 73.07 | 0.25 | 0.6 | |
| Pseudo 13 | 74.56 | 0.11 | 74.53 | 0.06 | 0.23 | 75.59 | 0.03 | 75.43 | 0.20 | 0.22 | 72.94 | 0.27 | 72.61 | 0.15 | 0.53 | |
| B | Patient 2 (HCC) | 74.09 | 0.12 | - | - | 0.47 | 73.15 | 0.06 | - | - | <0.0001 | 72.02 | 0.17 | - | - | 0.0086 |
| Patient 5 (HCC) | 73.74 | 0.07 | - | - | 0.03 | 73.22 | 0.06 | - | - | <0.0001 | 71.95 | 0.07 | - | - | 0.0002 | |
| Patient 6 (HCC) | 73.78 | 0.02 | - | - | 0.03 | 74.36 | 0.21 | - | - | 0.001 | 71.93 | 0.16 | - | - | 0.0028 | |
| Patient 9 (HCC) | 73.64 | 0.03 | - | - | 0.015 | 73.26 | 0.11 | - | - | <0.0001 | 72.10 | 0.11 | - | - | 0.0046 | |
| HCC Average | 73.81 | 0.00 | - | - | 0.0053 | 73.50 | 0.11 | - | - | <0.0001 | 72.00 | 0.05 | - | - | <0.0001 | |
| Patient 1 (CHB) | - | - | 74.12 | 0.17 | - | - | - | 75.74 | 0.18 | - | - | - | 72.13 | 0.08 | - | |
| Patient 3 (CHB) | - | - | 74.15 | 0.22 | - | - | - | 74.50 | 0.70 | - | - | - | 72.45 | 0.28 | - | |
| Patient 4 (CHB) | - | - | 74.19 | 0.27 | - | - | - | 75.56 | 0.03 | - | - | - | 72.25 | 0.01 | - | |
| Patient 8 (CHB) | - | - | 74.33 | 0.07 | - | - | - | 75.67 | 0.08 | - | - | - | 72.69 | 0.25 | - | |
| CHB Average | - | - | 74.20 | 0.18 | - | - | - | 75.36 | 0.10 | - | - | - | 72.38 | 0.01 | - | |
The value for every patient in Chinese cohort was compared with the average of normal adjacent tissue in 7 non-invasive patients (NorAdjRef, non-PVTT average). The value for every patient in Bangladeshi cohort was compared with the average of all CHB samples.