Figure 2. IL-10 GFP⁺ B-cells reduce cerebral cell infiltration post-MCAO.

Forty-eight hours after MCAO, mononuclear cells were isolated from brains of RPMI and IL-10 GFP⁺ B-cell recipient mice and were analyzed for A. Total cell count via hemocytometer. Values represent mean numbers (±SEM) of indicated cell subsets from 10–11 mice per group, from 3 separate experiments. B. CD3⁺ T-cells. C. CD11b⁺CD45^high activated microglia (MG)/monocytes, and D.CD11b⁺CD45^low resting MG obtained from the non-ischemic (left) and ischemic (right) hemispheres of µMT^−/− recipient mice. B–D, Values represent mean numbers (±SEM) of indicated cell subsets, gated on live leukocytes (by PI exclusion), from 4–5 mice per group, from at least 2 separate experiments. Statistical analysis was performed with ANOVA followed by Neuman-Kuels post hoc test. Significant differences between sample means are indicated (*p≤0.05; **p≤ 0.01; ***p≤ 0.001).