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. Author manuscript; available in PMC: 2014 Aug 1.
Published in final edited form as: J Neurochem. 2013 Jun 10;126(4):462–472. doi: 10.1111/jnc.12309

Figure 1. Kv4.2(W8A,F11A) mutant selectively disrupts regulation by KChIP3 but not DPP6.

Figure 1

A) Expressed currents for CHO-K1 cells transfected with the combination of subunits indicated. Kv4.2 expression is enhanced by both KChIP3 and DPP6. Kv4.2(W8A,F11A) expression is only enhanced by DPP6, not KChIP3. B) Summary data for currents in response to a depolarizing voltage step to +50 mV recorded by whole-cell voltage clamp from CHO-K1 cells. Kv4.2 and Kv4.2(W8A,F11A) expressed alone produce low levels of A type K current that are not significantly different. Co-transfection with KChIP3 cDNA significantly boosts the functional expression of Kv4.2 by 30 fold with no significant change in the functional expression of Kv4.2(W8A,F11A). Kv4.2(W8A,F11A), however, is regulated by another auxiliary subunit protein DPP6 with no significant difference to Kv4.2. C) Both Kv4.2 and Kv4.2(W8A,F11A) subunits show co-assembly with DPP6 in co-immunoprecipitation studies. Myc-tagged DPP6 was co-transfected with either Kv4.2 or Kv4.2(W8A,F11A). Lysate loading control lane (L) contains 1/5 of the amount of material used in each IP reaction. Antibodies used in precipitations shown above the lane. Anti-myc western to detect DPP6 shows that precipitation of either wild type or mutant Kv4.2 subunits co-precipitates DPP6 (Kv4.2-Ab lanes).