Figure 7. Visualization and study of the in vivo outgrowth of cancer cells isolated from blood using the FMSA device. Various amounts of GFP-labeled HCT-116 cells (indicated on left y-axis) were spiked into 7.5 mL blood and implanted into the right flank of hairless SCiD mice (A) Pseudo-color fluorescent imaging of FMSA-derived tumors at day 14 (B) axial/transverse sections of FMSA-derived tumors taken with ultrasound imaging at day 14 (C) calculation of tumor microvasculature perfusion percentage using ultrasound imaging and micro-bubbles at day 14 (D) excised FMSA-derived tumors (cm scale) at day 30 (E) Ki-67 immunohistochemical staining of FMSA-derived tumors.