Figure 4. miR-125b and miR-34a have divergent effects on the p53/miR-34a/SIRT1 signaling network in multiple myeloma cells. (A) qRT-PCR analysis reveals enhanced p53 and repressed SIRT1 mRNA expression in MM.1S and MM.1R cells following transfection with anti-miR-125b-transcripts or synthetic miR-34a-mimics. (B) Transfection with anti-miR-34a-transcripts or synthetic miR-125b-mimics reveals an antagonistic effect. (C) Western blot analysis of total p53 and SIRT1 protein expression correlate with mRNA in response to anti-miR-125b or miR-34a-mimics in MM.1S and MM.1R cells. Values indicate the mean ± SEM from three independent experiments (statistical significance calculated by Student’s t-test; *, indicates p ≤ 0.05).