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. 2013 Feb 27;10(5):792–802. doi: 10.4161/rna.24023

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Copyright © 2013 Landes Bioscience

This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.

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graphic file with name rna-10-792-g2.jpg — Figure 2. Distribution in pCas1-2(K) of sequences matching distinct spacers sampled in acquisition assays. Spacers are shown as black arrows pointing toward the leader as they were inserted in the integration cassette. The origin of replication (CDF ori), the genes encoding the lac operon repressor (lacI) and a streptomycin resistance protein (SmR) as well as the T7-lac promoter (P_T7) leading transcription of K-variant cas1-2 genes (cas1-K and cas2-K) are shown.