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. 2013 Aug 7;8(8):e70422. doi: 10.1371/journal.pone.0070422

Figure 1. Schematic representation of Tiam1 constructs and expression level of Tiam1 in RB tumors compared to normal retina.

Figure 1

A. Full length Tiam1 consists of 1591 aminoacids. Tiam1 has several specific domains such as, Myr: Myristoylation site; P: PEST sequences; PHn: N-terminal PH domain; CC: Coiled-coil region; Ex: Extended structure; RBD: Ras-binding domain; PDZ: PSD-95/DlgA/ZO-1 domain; DH: Dbl homology domain and PHc: C-terminal PH domain. Deletion of N-terminal myr and P sites leaves the active C1199 form of Tiam1 in truncated constructs. C580 contains only DH and C-terminal PH domain. B. Fold difference in the expression of Tiam1 mRNA in RB tumors versus normal retina by qPCR analysis. The relative fold change (log2 ratio) in Tiam1 mRNA expression is calculated by normalizing with β2-microglobulin using normal retina as control sample. C. Western blot analysis of Tiam1 protein expression in RB tumors and normal retina shows Tiam1 at 180 kda and β-actin at 42 Kda. D. Densitometry analysis of the Tiam1 protein expression in RB tumors vs normal retina (n = 2). RB1 to RB7 invidividual expression normalized with averaged NR is represented.