Skip to main content
. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: Nat Med. 2013 Jul 7;19(8):1054–1060. doi: 10.1038/nm.3259

Figure 6.

Figure 6

Notch induces mTorc1 complex stability. (a) Western blots of liver proteins from 5h-fasted L-Rbpj and control mice (mTor and Actin blots reproduced from Figure 2i). (b) Western blot of liver protein from chow-fed, 12-week-old C57Bl/6 male mice transduced with Fc or N1-IC, sacrificed at day 7, after overnight fasting. (c) Western blots from FAO hepatoma cells transduced with either Fc or N1-IC, with or without treatment with MG132 for 4-h. (d) Western blots of primary hepatocytes, transfected with Raptor cDNA, then transduced with Fc or N1-IC and treated for 2-h with cycloheximide. (e) Fasn-luciferase activity in FAO hepatoma cells transfected with Fasn-luciferase reporter, co-transduced with Fc or N1-IC and GFP or Raptor. 24-h after transduction, hepatoma cells were transferred to serum-free medium for 16-h, then treated with 10 nM insulin for 6-h prior to lysis. ***p<0.001 vs. Fc. Data show means ± SEM. (f) Western blots of HEK 293 cells or (g) primary hepatocytes transfected with Raptor-FLAG, followed by transduction with GFP or N1-IC and immunoprecipitation with anti-FLAG antibody. Protein expression normalized to either actin or tubulin. (h) Model of Notch effects on hepatic glucose and lipid metabolism.