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SPR analysis of the binding of the asialoglycoproteins with immobilized galectin-3. (A) de-sialylated SGP starting concentration at 800 μM with 1:2 serial dilutions; (B) asialofetuin, starting concentration at 10 μM with 1:2 serial dilutions; (C) HSA-Gal15, starting concentration at 6 μM with 1:2 serial dilutions; (D) HSA-Gal30, starting concentration at 250 nM with 1:2 serial dilutions. Inset, fitting of the SPR data.
