Fig. 1.

Construction of linear fragments for transforming S. mutans. Transformation was carried out as described previously (13). Numbers depicted above and below the fragments (fk), (zJ), (zK), and (ae) correspond to the nucleotide positions on the complementary strand of the UA159 genome. Ovals in the figure indicate promoter regions, which are abbreviated as ‘prom’ containing a 114-bp intergenic region between the malR and malQ genes. A MalR-binding consensus sequence is located between the -35 and -10 regions of a putative promoter sequence. CTC in the fragment (ae) are added nucleotides for splicing the fragments and are located immediately upstream from the Shine–Dalgarno sequence of the glgP gene.