Fig. 4.

Thin-layer chromatography (TLC) analysis. An aliquot (2.5 µl) of the reaction mixture incubated for 100 min containing products from the activity of the purified MalQ enzyme was spotted at lane 4 next to the control (lane 3) spotted with a 1 µl aliquot of enzyme reaction mixture before incubation. Lanes: 1, 0.5 µl of 1% glucose; 2, 1 µl of 1% maltotriose; 5, 1 µl of 1% maltopentaose; 6, 1 µl of 1% maltodextrin. Arrows indicate glucose, maltose, maltotriose, maltotetraose, and maltopentaose. Abbreviations: Bf.RwMaltose, before reaction with maltose; Af.RwMaltose, after reaction with maltose.