Table 1.
Primers used in this study
| Primer designations | Sequences (5′>3′) | Purpose or target region |
|---|---|---|
| exmalQ5 | ATCTCGAGATGGAAAAACGCGCAAGTGG | MalQ expression |
| malQ32 | TGGACATACTTACTGAATTGTTTTGTTTTT | MalQ expression |
| expglg51 | ATCTCGAGGAAATGAAGAAAATGACACAAAA | GlgP expression and glgP5′ in (ae) |
| expglg31 | CTTTTTACAAACTATGCCAAATGTCC | GlgP expression |
| malR53 | GGGATCCTGTACGTCAATATCTGA | malR3′ in (ae) |
| EmTailmalR3 | CACACTCTTAAGCTAGCTTTTTTTAAGATTACGGACA | malR3′ in (ae) |
| malRTailEmF | TAAAAAAAGCTAGCTTAAGAGTGTGTTGATAGTGCAGT | Emr in (ae) |
| IGTailEmR | AAGCTTCTAATCTCTAGGGACCTCTTTAGCTCCT | Emr in (ae) |
| EmTailmalQ5 | AGGTCCCTAGAGATTAGAAGCTTAATTTCTAAGCTTTT | Promoter region in (ae) |
| XhglgTailIG3 | TTCTTCATTTCCTCGAGATTTTCCATATAGTCTCCTCGCT | Promoter region in (ae) |
| glg31 | AACATAAAGAGCATTCATTTGCTG | glgP5′ in (ae) & glgP3′ in (zK) |
| expglg52 | ATCTCGAGAATGACACAAAAAACAAAACAATTCAG | glgP5′ in (zK) |
| kanTailglg3 | GGTTTATCCGGGATCCGTCAAGCCATAATCAAGACCATCA | glgP5′ in (zK) |
| kanF | GGATCCCGGATAAACCCAG | kanr in (zK) |
| kanR | GCGGATCCCGAGCTTTT | kanr in (zK) |
| kanTailglg5 | AAGCTCGGGATCCGCTGGCTGATTATGCCTATGTG | glgP3′ in (zK) |
Underlined sequences are those of added nucleotides for restriction endonuclease reactions or those necessary for the splicing by the overlapping extension method.