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Edelfosine, W30, and R59949 can have similar effects on basal gene expression. We verified on a set of genes that edelfosine, wortmannin, and R59949 could, separately, stimulate or repress basal expression. Cells were treated with edelfosine, wortmannin, or R59949 for 4 h, at 22°C, before harvesting. Transcript levels were estimated by reverse transcriptase-PCR, using an appropriate number of cycles.
