Figure 6.

Effects of inhibitors of PI-PLC or DGK on the expression levels of DREB2 genes in seedlings. Twelve-day old plants grown in liquid medium under continuous light were incubated with inhibitors and harvested at desired times. (A) Plants were treated with 100 M edelfosine. Transcript levels were estimated by reverse transcriptase-PCR, using an appropriate number of cycles. (B) Plants were treated with 100 M R59022. Transcript level were measured by qPCR, and expressed as % of the level in solvent treated plants at the desired time.