Figure 2.

Effect of Ins11R/S12H and Del25 in cell-cell fusion assay (a). Cell-cell fusion assay was performed using Env-expressing 293 T cells and CD4⁺ and CCR5⁺/CXCR4⁺ COS-7 cells. Data are mean±SD values in relative luminescent unit (RLU) of six experiments (performed in duplicate and repeated three times). Analysis of two elements of Ins11R in cell-cell fusion assay (b and c). Effects of placing R at position 11 (b) and one amino acid elongation of V3 (c) were analyzed. Cell-cell fusion assay was performed using Env-expressing 293 T cells and CD4⁺ and CCR5⁺/CXCR4⁺ COS-7 cells. Data are mean±SD values in relative luminescent unit (RLU) of six experiments (performed in duplicate and repeated three times).