Figure 4.

Effects of T8V in cell-cell fusion assay (a and b). The effects of T8V were analyzed in combination with Ins11R/S12H/Del25 (a), and Del25 and Ins11R/S12H (b). Cell-cell fusion assay was performed using Env-expressing 293 T cells and CD4⁺ and CCR5⁺/CXCR4⁺ COS-7 cells. Data are mean±SD values in relative luminescent unit (RLU) of six experiments (performed in duplicate and repeated three times). Tropism of recombinant HIV-1 variants harboring mutations identified in Case 1 (c). Tropism of HIV-1 variants was assessed in CCR5⁺ GHOST Hi5 and CXCR4⁺ GHOST CXCR4 cells. The mean fluorescent intensity (MFI) of infected cells expressing green fluorescent protein (GFP) was measured. Data are mean±SD values of six experiments (performed in duplicate and repeated three times).