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. 2013 May 17;41(14):7060–7072. doi: 10.1093/nar/gkt414

Figure 1.

Figure 1.

Thoc5 interacts with pre-mRNA cleavage and polyadenylation factors. (A–F) RNase A-treated HeLa whole-cell extract was subjected to immunoprecipitation using the antibodies indicated on top of each lane. (A) The immune pellets were subjected to SDS–PAGE, followed by CBB staining (left) or western blotting using the indicated antibodies (right). The position of the 70-kDa protein is indicated with arrowheads. The asterisks indicate the position of IgG heavy chain. (B–F) The immune pellets were subjected to western blotting using the antibodies indicated at the right of each panel. In (B), the position of CFIm68 is indicated with an arrowhead. The anti-CFIm68 antibody also cross-reacts with CFIm72 and CFIm59 (indicated with curved arrows), which are detected as slower and faster migrating bands, respectively (14).